Blyscan Glycosaminoglycan Assay

Glycosaminoglycan Assay Blyscan™


The Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).

Test material can be assayed directly when present in a soluble form, or following papain extraction from biological materials.  The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.

The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.

The assay is not suitable for small sulfated disaccharide fragments or for the samples containing alginates, as these contain uronic acid.