Sircol™ COLLAGEN Assay

The Sircol™ Assay is a quantitative dye-binding method for the analysis of acid-soluble collagens extracted from mammalian tissues and collagens released into culture medium by mammalian cells during in vitro culture.

Salt-soluble, acid-soluble and pepsin-soluble forms of mammalian collagens, Types I to IV, can be measured.

The pictures below describe this simple three-step assay:

Mix sample and dye reagent

Centrifuge sample and decant unbound dye

Retain collagen bound dye

Recover bound dye using Sircol dye release reagent

Measure colour absorbance in cuvette or microwell plate

* Pictures obtained using the Sircol kit; range of 0-50 μg collagen

Assay time: 1 hour Assay sensitivity: 2.5µg

Sircol™ Collagen Assay Kits:

Standard assay kit (120 assays) ..................Product Code: S1000

Large economy assay Kit (475 assays).........Product Code: S5000

Components of the S1000 kit:

Sircol Dye reagent (120 ml);
Alkali Dye Release reagent (120 ml);
Salt-soluble Collagen Precipitating reagent (12.5 ml);
Acid-soluble Collagen Standard (5 ml of 1.00 mg/ml);
Sircol Assay Manual

Dye Reagent Pack [3 x 120ml]..................Product Code: S1005

Components of the S1005 kit:

3 x Sircol Dye reagent (120 ml)

Acid-soluble Collagen Standard [3 x 5ml]..................Product Code: S1010

Components of the S1010 kit:

Sterile ampoules of acid-soluble collagen (1.00mg/ml)