Elastin Assay – Fastin™ Elastin


The Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.

The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).

Elastin forms that can be measured by the Fastin Assay as α-elastin:

  1. soluble tropoelastins;
  2. lathyrogenic elastins;
  3. insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])

The dye reagent binds to the ‘basic’ and ‘non-polar’ amino acid sequences found in mammalian elastins.  For analysis of soluble elastin, samples should be free of any particulate material (cell debris, insoluble extracellular matrix material).  The presence of other soluble proteins or of complex carboyhdrates does not interfere with the Fastin Assay.