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Blyscan™ GLYCOSAMINOGLYCAN Assay

Frequently Asked Questions

Q. What is the sensitivity of the Blyscan assay?
A. 0.5μg

Q. How long does the Blyscan assay take to run?
A. 1 hour

Q. Can I differentiate between N-sulfated and O-sulfated glycosaminoglycans?
A. A method is given in the manual to differentiate between the glycosaminoglycans present and all necessary reagents are supplied in the kit.

Q. What absorbance values can samples be measured at?
A. The absorbance peak for Blyscan dye in the dissociation reagent is 656nm. This absorbance is suitable for use with most colorimeters and microplate readers with a red filter.

Q. How do I prepare samples for measurement with the Blyscan assay?
A. Preparation details for samples in tissue culture medium, cartilage/soft tissue and urine samples are described in the assay manual.

The Blyscan assay manual is the primary source of further information. It may also be helpful if the user familiarises themselves with some of the research papers in which the Blyscan assay has been cited. Click the links below to download a preview copy of the assay manual, and a comprehensive list of Blyscan citations.

Blyscan Updated Manual Blyscan Citations