Fastin™ ELASTIN Assay

The Fastin™ Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.

Elastin forms that can be measured:

  1. soluble tropoelastins;
  2. lathyrogenic elastins;
  3. insoluble elastins (as solubilised elastin polypeptides [α-elastin, κ-elastin])
    Furthermore:
  4. elastase activity can be measured, using elastin as an enzyme substrate

The pictures below outline the Fastin™ Assay:

Biocolor Fastin Assay:Cold precipitated elastin [1] Cold precipitated elastin (1 mg, overnight), centrifuged and drain dried
Biocolor Fastin Assay:Elastin-dye complex after cenfrifuging and removing unbound dye. [2] Elastin-dye complex after cenfrifuging and removing unbound dye.


Left: Reagent blank
Right: Standard (50 µg)
Biocolor Fastin Assay:Recovery of elastin bound dye, after adding dissociation reagent and centrifugation [3] Recovery of elastin bound dye, after adding dissociation reagent and centrifugation


Left: Solvent/buffer blank
Middle: Reagent blank
Right: Standard (50 μg)

Assay time: 4 hours     Assay sensitivity: 0.5µg

Fastin™ Elastin Assay Kits

Standard Assay Kit (120 assays)…………………Product Code: F2000



Large economy Assay Kit (475 assays)…………..Product Code: F4000
Components of the F2000 kit:



Fastin Dye reagent (120 ml);
Precipitation reagent (120 ml);
Dye Dissociation reagent (120 ml);
α-Elastin Standard (5ml of 100 μg/ml);
Fastin Assay Manual
Dye Reagent Pack [3 x 120ml]…………………..Product Code: F2300
Components of the kit:


3 x 120ml Fastin Dye reagent
α-Elastin Standard [3 x 5ml]…………………..Product Code: F2010
Components of the kit:



Sterile ampoules of α-elastin (100μg/ml)
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