APOPercentage™ Apoptosis Assay

Frequently Asked Questions

Q. What is the sensitivity of the APOPercentage assay?

A. A Single apoptotic cell within a microwell cell population.

Q. How long does the APOPercentage assay take to run?

A. 1 hour

Q. What types of cell can the assay be used with?

A. Established cell lines are suitable. Cells should be anchorage-dependent. In order to enhance attachment the cells exposed to toxic agents may be grown in the gelatin included with the kit.

Q. How are the results quantified?

A. The dye used is purple-red in colour and can be detected with a conventional inverted microscope, as used in cell culture laboratories, at magnifications of x100 and x200. With an attached digital camera photomicrographs can be obtained and transferred to a computer. These images may then be used for further analysis and quantification by Analytical Digital photomicroscopy (ADP*). The dye that accumulates within labelled cells can also be released into solution and the concentration of intracellular dye measured using a microplate colorimeter or fluorimeter.

Q. What absorbance values can samples be measured at?

A. 550nm on a microplate colorimeter

or 530nm (excitation) and 580nm (emission) with a microplate fluorimeter.

The APOPercentage assay manual is the primary source of further information. It may also be helpful if the user familiarises themselves with some of the research papers in which the APOPercentage assay has been cited. Click the links below to download a preview copy of the assay manual, and a comprehensive list of APOPercentage citations.

APOPERCENTAGE APOPTOSIS ASSAY MANUAL