Suitable for monitoring collagen production during in-vitro cell culture or of recent in-vivo synthesis from tissue samples; such as wound healing and skin grafts research. Fibrillar collagens, [type I, II, III, V & XI] and basement membrane collagen, [type IV]. assay for acid-soluble, (cold 0.5 M acetic acid), mammalian collagens.
Assay sensitivity; 2.5 μg Assay run time; 1 hour
For analysis of sulfated glycosaminoglycan components of proteoglycans including decorin, biglycan, fibromodulin, aggrecan, syndecan, betaglycan. Galactosaminoglycans: chondroitin sulfates, dermatan sulfate. Glucosaminoglycans: heparan sulfate, heparin, keratan sulfate. Test material; cell culture medium, amniotic fluid, urine, synovial fluid and tissue extracts.
Assay sensitivity; 5 μg Assay run time; 1 hours
Suitable for in-situ tissue insoluble cross-linked elastin as found in elastic cartilages and in soft tissues, such as the aorta and skin. Elastin produced during in-vitro cell culture. Hot oxalic acid extraction is required prior to analysis to bring the elastin into solution.
Assay sensitivity; 0.5 μg Assay run time; 4 hours
