Blyscan™ GLYCOSAMINOGLYCANS Assay

The Blyscan™ Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans.

Sulfated glycosaminoglycan (sGAG) forms that can be measured; soluble salt extracts from:

  1. in vitro studies where extracellular matrix components are released by live cells into the cell culture medium;
  2. elastic, fibrous and hyaline cartilages;
  3. arteries, lung, skin and other material containing extracellular matrix (connective tissue);
  4. solid tumour specimens;
  5. cell and tissue extracts;
  6. aliquots from protein chromatography fractions
     
    Furthermore:
     
  7. sulfated glycosaminoglycans in amniotic fluid and urine samples can be measured;
  8. the assay may be adopted to detect and/or measure sulfated glycosaminoglycan degradative enzymes, and;
  9. the N- and O-sulfated glycosaminoglycan ratio can be measured

The pictures below outline the Blyscan™ Assay:

Blyscan Assay Step 1

[1] Reagent blank (left) and Reference standard (right), mixed with 1 ml Blyscan Dye reagent
Note: Pink 'blush' in the Reference standard tube

Blyscan Assay Step 2

[2] Tubes centrifuged and supernatant removed
Note: Purple pellet of dye-sGAG complex in Reference standard tube

Blyscan Assay Step 3

[3] Tubes after addition of the Blyscan Dye Dissociation reagent
Note: The pink/purple colour of the dye-sGAG complex has been replaced by the blue colour of the “free” dye

Assay time: 1 hour     Assay sensitivity: 0.5µg

Blyscan™ Sulfated Glycosaminoglycan Assay kits

Standard assay kit (120 assays)................Product Code: B1000

Large economy assay kit (475 assays)......Product Code: B3000

Components of the B1000 kit:

Blyscan Dye reagent (120 ml); Dissociation reagent (120 ml);
Glycosaminoglycans Standard (5 ml of 100 µg/ml Ch-4-SO4);
Nitration reagents (15 ml);
Blyscan Assay Manual

Dye reagent & Dissociation reagent pack.....Product Code: B0500

Components of the kit:

2 x 120ml Blyscan Dye Reagent, plus
2 x 120ml Blyscan Dissociation reagent

Dye reagent pack [3 x 120ml]......................Product Code: B1005

Components of the kit:

3 x 120ml Blyscan Dye reagent

Glycosaminoglycan Standard [3 x 5ml].......Product Code B1010

Components of the kit:

Sterile ampoules of chondroitin-4 sulphate (100 µg/ml)

sGAG Isolation & Concentration pack..........Product Code B1015

Components of the kit:

Buffered cetylpyridinium chloride and 2 M lithium chloride:
to clean-up and concentrate test samples, prior to assay;
also for the pre-treatment of test samples with GAG level of less than 5 µg/ml
Sufficient reagents for 100 samples