APOPercentage™ APOPTOSIS Assay

The APOPercentage™ Assay is a detection and MEASUREMENT system to monitor the incidence of apoptosis in mammalian, anchorage dependent, cells during in vitro culture.

Apoptotic cells take up the APOPercentage dye, as shown in the pictures below.

APOPercentage Stages

Digital images of CHO cells treated with 10 mM cycloheximide, to induce apoptosis after 2, 4 and 6h

Following dye uptake, the level of apoptosis can be quantified colorimetrically, fluorometrically or by analytical digital photomicroscopy (ADP*).

Assay time: 1 hour     Assay sensitivity: a single apoptotic cell.

APOPercentage™ Apoptosis Assay Kits:

Standard Assay Kit, 500 assays (6 x 96 wells; microwell format)......Product Code: A1000

Components of the kit:

Components of the A1000 kit:
APOPercentage Dye reagent (5 ml) in phosphate buffered saline (sterile vial);
APOPercentage Dye Reference Standard (10 µM/10 ml), (sterile vial);
APOPercentage Dye Release reagent (150 ml);
Gelatin matrix forming solution (0.4%/100 ml / sterile);
APOPercentage Assay Manual