APOPercentage™ APOPTOSIS Assay

Apoptosis Detection

APOPercentage FAQs


The APOPercentage™ Apoptosis Assay is a detection and MEASUREMENT system to monitor the incidence of apoptosis (programmed cell death) in mammalian, anchorage dependent cells during in vitro culture.


The following pictures are digital images of CHO cells exposed to cycloheximide (10mM) for 2, 4 and 6 hours.


CHO cells treated with Biocolor ApoPercentage Dye Reagent

Cycloheximide induces apoptosis thus enabling the cells to take up the APOPercentage dye.

Following dye uptake, the level of apoptosis can be quantified colorimetrically, fluorometrically or by analytical digital photomicroscopy (ADP*).

The level of apoptosis can be measured and expressed as a pixel number using GIMP (the GNU Image Manipulation Program) or Adobe Photoshop software.

Download GIMP

Download GIMP program

 

Time required -  1 hour.     Detection Limit – a single apoptotic cell.


APOPercentage™ Apoptosis Assay Kit

Standard Assay Kit, 500 assays (6 x 96 wells; microwell format)


Product Code: A1000

Components of the A1000 kit:
APOPercentage Dye reagent (5 ml) in phosphate buffered saline (sterile vial);
APOPercentage Dye Reference Standard (10 µM/10 ml), (sterile vial);
APOPercentage Dye Release reagent (150 ml);
Gelatin matrix forming solution (0.4%/100 ml / sterile);
APOPercentage Apoptosis Assay Manual

Click to Order

Apoptosis Detection

Print This Page Print This Page

Get Cheap Cell Phones and Wireless Deals. | Thanks to Best Savings Accounts, CD Rates and UK Loan